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Zeiss Lattice Lightsheet 7
Zeiss Lattice Lightsheet 7
What's so interesting?
What's so interesting?
The Zeiss Lattice Lighsheet 7 (LLS7) is optimized for rapid live-cell imaging in four dimensions (x, y, z and time).
The system is capable of high-speed volumetric imaging, and produces high signal-to-noise ratio images with minimal photobleaching.
It is equipped with 2 Hamamatsu ORCA-Fusion cameras for 2-color simultaneous imaging, three solid-state lasers ( 488, 561, 640nm), an Ibidi environmental chamber with temperature, humidity, and C02 control, and a motorized x-y-z/tilt stage.
Suitable for adherent cells, spheroids, organoids, and small evolving organisms.
New Users
New Users
Faculty and students who wish to use the microscope should follow the instructions in the New Users Guide. Training takes ~2 to 3 hrs, with additional mandatory follow-up sessions. Users are encouraged to bring their own samples to the training session. If none are available, prepared slides will be used.
Policy
Policy
The microscope is available to trained users on an equal basis. An individual user may reserve one four hour block of time during "peak" daytime hours, and an additional 6 "off-peak" hours (all other times). Investigators performing live cell imaging experiments may signup for 12-14 hours blocks of time after 6pm.
For a comprehensive list of policies (including BSL-2 protocols), please see the Facility SOP.
Rates
Rates
The rate schedule applies to different users and is current for the 2024 academic year. Fees are used to help cover the cost of the service contract on the microscope, and to help pay for materials such as lens paper, lens cleaning solution and mercury bulbs.
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